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连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的前切位点以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性如果有同裂酶存在，则只列出其中的•个前本表中列出的内切酶均为已商品化的内切酶识别序列不够严谨的内切酶（例如，识别多个序列的陶）只在括号内标出相关的序列，但注意这些酶不只切割该序列表示连接产物不能被再切割酶连接到可进行切割的酶Ban1G/GTACC Acc65I,Ban1,Kpn1,NiaIV,Rsa1Acc65lG/GTACCBsiW1,BsrG1Rsa1Acc1GT/CGAC AciLAcl1,BsaH1GR^CGYC,HinP1I,HpaII,Nar1—GT/CGAC Cla1,BstB1,Taq1Taq1Aci1C/CGC Acc1GT/CGAC,Acl1,Cla1,BstB1,Taq1-BsaH1GR/CGCC,HinP1I,Nari AcilHpaIIHpaIIAccIGT/CGAC,AciI.Cla1,BstB1,HinP!I,HpaII,Nar1,Taq1Acl1AAJCGTT-AfiIIIAfiIIA/CATGT,BspH1,Neo1,Sty1C/CTAGG NiaIIIPci1A.CATGTPpuM1RG/GWCCY AvaII,Rsrll AvaII.Sau96IGG/GTCCY AvaII,RsrII AvaII,BsmF1,NiaIV.Sau961GG/GACCY AvaII.RsrII AvaII.NiaIV.Sau961PspOM1G/GGCCCEae1Y/GGCCR,Eag1HaeIII,Sau961Notl Aci1,Fnu4HLHaeIII.Sau961PstlCTGCA/GBsiHKA1,Bsp1286IGTGCAC BsglNsiI—Sbfl PstlPvu1CGAT/CG AsiSIDpn1,Pvu1PacI Mse1BsiEICGAT/CG BsiE1,DpnII,Pvu1RsrllAvaII,PpuMIRG^GACCY AvaII.Sau961CG/GWCCGPpuMIRG/GACCY AvaII.NiaIV.Sau961PpuMIRG/GTCCY AvaII.BsmF1,NiaIV,Sau961BanIIGAGCT/C.BsiHKAI,Bsp1286IGAGCT/C Alu1,BanII,BsiHKA1,Bsp1286I,SacISac1GAGCT/CBsiEICGGC/CGSacIICCGC/GG AciIAva1C/TCGAG,Xho1Taq1Sal1G/TCGACSbtlCCTGCA/GGBsiHKA1,Bsp12861GTGCAC Bsg1Nsi1-Pstl PstlApaL1Bsg1Sfclc.qrGCAGSeeBsrF1SgrAICR/CCGGYGAvrII,Nhe1,Sty1C/CTAGG,Xba1BfalSpe1A/CTAGTNiaIII,Nsp1NiaIIISphlGCATG/CStyl AvrilAvrII,Bfa1,BsaJ1,Sty1C/CTAGG Nhe
1.Spe1,Xba1BfalC/CATGG BspH1Nia川Neo1BsaJ1,Neo1,NiaIILSty1Acc1GT/CGAC,Cla1,BstB1Taq1Taq1T/CGAAci1,Acl1,BsaH1GR/CGYC,HinP1I.HpaII,NarI-Tsp5091/AATT Apo1R/AATTY,EcoR1,Mfe1Tsp5091AvrII,Nhe1,Spe1,Sty1C/CTAGG BfalXbalT/CTAGAXhoimi1Ava1C/TCGAG Aval,Taq1,Xho1C.TCGAG SallTaqlXma1Age1,BsaW
1.BspE1,BsrF1,NgoMIV,SflrA1HpaII,Nci1,ScrFIC/CCGGG Ava1C/CCGGG Ava1,BsaJ1,HpaII.Nci1,ScrF1,Sma
1.Xma1A.CGCGT Asc1,BssHII BstU1,Hha1A/CATGT BspH1,Neo1,Pcil NiaIIIA/CGCGT Miu1AfiIII,BstU1,Miu1AgelA^CCGGTAva1C/CCGGG,Xma1HpaII,Nci1,ScrFIBsaW
1.BspE1BsaW1,HpaIIBsrFIA.CCGGT,SgrA1CA/CCGGTG Age1,BsaW
1.BsrF1,HpaIINgoMIV BsrF1,HpaIIBanIIGGGCC/C,Bsp12861GGGCC/C Apa1,BanII,Bsp1201,Bsp1286I,HaeIII,NiaIV,Apa1GGGCC/CSau96ISfc1C/TGCAG BsglApaLIG/TGCACApo1A^AATTY EcoR1ApoI,Tsp509IG/AATTY EcoRIApoI,EcoRI,Tsp5091R/AATTY Mfe1,Tsp5091Tsp5O91Asc1GG/CGCGCCAfiIIIA/CGCGT,Miu1BstU1,Hha1BssHII BssHII.BstU1,Cac81,Hha1Ase1AT/TAATBfa
1.Csp61,Nde1-Mse1Mse1AsiSIGCGAT/CGCBsiE1CGAT/CG DpnII,Pvu1Pad MselPvu1DpnII,Pvu1Ava1C.CCGGG Age1,BsaW1,BspE1,BsrF1R/CCGGY,NgoMIV,SgrA1HpaII,Nci1,ScrFICR/CCGGYGC.G-CGAG XholAva1,Taq
1.Xho1C.nrCGAG Sal1Taq1Xma1Ava1,BsaJ1,HpaILNci1,ScrF1,Sma1C/CCGGGAvaIIPpuM1RG/GACCY AvaII,NiaIV,Sau961G/GWCCRsrllAvaII.Sau961PpuM1RG/GTCCY AvaII.BsmF1,NiaIV.Sau961AvrII Nhe1,Spe1,Xba1BfalC/CTAGGSty1C/CTAGG AvrII,Bfa1,BsaJ1,Sty1BamH1G/GATCCBelLDpnII Alw1,DpnIIBglII,BstY1R/GATCY Alw1,BstY1,DpnIIBstY1G/GATCC Alw1,BamH1,BstYI,DpnII,NiaIVBan1G/GTACC ACC65I Acc
651.Ban1,Kpn1,NiaIV.Rsa1G/GCGCC Kas1Ban1,BsaH1,HaeII,Hha1,Kas1,Nar1,NiaIVG/GTACC BsiW1,BsrG1Rsa1BanIIGGGCC/C Apa1,Bsp1286IGGGCC/C Apa1,BanII,Bsp1286I,HaeIII,NiaIV,Sau961GAGCT/C Bsp12861GAGCT/C,Sac1Alu1,BanII,BsiHKA1,Bsp1286I,Sac1BellT/GATCABamH1,BstY1R/GATCY Alw1,DpnIIBglII,MboII DpnIIAse1,Csp61,Mse
1.Nde1BfalC/TAG-BglIIA.GATCTBamH
1.BstY1R/GATCY Alw1,BstY1,DpnIIBel1,DpnII DpnIIBsaHIGR/CGYC Acc1GT/CGAC,Cla1,BstB1,Taq1—GA-CGYC Aci1,HinPlI Hga1GG/CGYC AciI,HinPlI HhalGG/CGYC HpaII AcilG^CGYC NarIBsaH1,Hga1GG/CGYC NariBan1,BsaH1,HaeII,Hha1,Nari,NiaIVBsaWIW/CCGGWAge1,BsrF1RCCGGY,SgrA1CR/CCGGYG Age1,BsaW1,BsrF1,HpaIIAva1C/CCGGG,Xma1HpaII.Nci1,ScrFIBspE1BsaW
1.BspE1,HpaIIBsrF1R/CCGGY,NgoMIV BsrFLHpaIINgoMIVHpaIIBsiEICGAT/CG PacIMselCGAT/CG PvuIBsiE1,DpnII,Pvu1CGGC/CG SacIIAcilBsiHKA1GTGCA/CBsp1286IGTGCAC BsiHKAl,Bsp12861Bsp1286IGAGCA/C BsiHKA1,Bsp12861Bsp12861GAGCT/C,Sac1Alu1,BanII,BsiHKA1,Bsp1286I,SacINsi1-Pstl,Sbf1BsglAcc651,Ban1G/GTACC,BsrG1RsaIBsiWIC/GTACGBsp1286IGGGCC/C Apa1,BanIIGGGCC/C ApaI,BanII,Bsp12861,HaeIII,NiaIV,Sau961GTGCA/C BsiHKA1ApaLI,BsiHKAl,Bsp1286lGGGCC/C BanIIGGGCC/C BanII,Bsp1286IGAGCT/C BanIIGAGCT/C,BsiHKA1,Sac1AluI.BanII,BsiHKAI,Bspl286I,SacIGWGCW/C BsiHKA1BsiHKAI,Bsp1286lGTGCA/C Nsi1-GTGCA/C Pstl,Sbf1BsqIBspEI Age1,BsaW1,BsrF1R/CCGGY,SgrA1CR/CCGGYG BsaWI,HpaIIT/CCGGAAva1C/CCGGG,Xma1HpaII,Nci1,ScrFIBsaWI BsaW1,BspE1,HpaIIBsrF1R/CCGGY,NgoMIV HpaIIAfiIIIA/CATGT,Neo1,Sly1C/CATGG NiaIIIBspHIT/CATGABsrFIA-CCGGY Age1,BsaW1Age1,BsaW1,BsrF1,HpaIIG/CCGGY Age1,BsaWLNgoMIV BsrFLHpaIIR/CCGGY Ava1C/CCGGG,Xma1HpaII,Nci1,ScrFIRCCGGY BsaW1,BspEI BsaWI,HpaIIR/CCGGY BsaW
1.BspE1HpaIIG/CCGGY NgoMIVBsrF
1.Cac81,HpaII,Nae1CR/CCGGYG SgrA1BsrF
1.HpaIIAcc651,Ban1G/GTACC,BsiW1Rsa1BsrG1T/GTACABssHIIAfiIIIA/CGCGT,Miu1BstU1,Hha1G^CGCGCAsc1BssHII.BstU1,Cac81,Hha1BslBIAcc1GT/CGAC,Cla1,Taq1Taq1TT/CGAAAci
1.Acl1,BsaH1GRQGYC,HinP1I,HpaII.Nari一BstYINGATCY BamHI,BglII Alw1,BstY1,DpnIIG/GATCY BamH1Alw1,BamH1,BstY1,DpnII,NiaIVR/GATCY Bel1,DpnII DpnIIG/GATCY Bel1,DpnII Alw1,DpnIINGATCY BglIIBglII.BstY1,DpnIIClalAcc1GT/CGAC,BstB1,Taq1Taq1AT/CGATAciLAcl1,BsaH1GFVCGYC,HinPlI,HpaII,Nari-BamHLBstYIR/GATCC Alw1,DpnIIDpnIVMboUSau3A1/GATCBelI,BglII,BstYIR/GATCY DpnIIEaelY/GGCCR PspOMIHaeIII.Sau961C/GGCCR Eag1BsiE1,Eae1,Eag1,HaeIIIT/GGCCR Eag1Eae1,HaeIIIC/GGCCR NotlAci1,BsiE1,Eae1,Eag1,Fnu4H1,HaeIIIT/GGCCR NotlAci1,Eae1,Fnu4H1,HaeIIIEaglC/GGCCGPspOM1HaeIII.Sau961Eae1Y/GGCCR Eae1,HaeIIIEae1C/GGCCG BsiE1,Eae1,Eag1,HaeIIINotl Aci1,BsiE1,Eae1,Eag1,Fnu4H1,HaeIIIApo1G/AATTC Apo1,EcoR1,Tsp5091EcoR1G/AATTCApo1R/AATTY Apo1,Tsp5091Mfe1,Tsp5091Tsp509IHinP11Acc1GT/CGAC,Acl1,Cla1,BstB1,Taq1-@CGCAci1,BsaH1GR/CGCC,Nari HhalBsaH1GR/CGTC HgaIHpaIIAciIHpaIL/Msp1Acc1GT/CGAC,Acl1,Cla1,BstB1,Taq1-C/CGGAciLBsaH1GR/CGCC,HinP11,Nar1AciIBan1G/GCGCC BanI,BsaHI,HaeII,HhaI,KasI,Nar1,NiaIVKaslG/GCGCCApo1R/ATTTY,EcoR1,Tsp5091Tsp5091MfelC/AATTGMIulAfiIIIA/CGCGT AfiIII.BstU1,Miu1A^CGCGTAsc1,BssHII BstU1,Hha1Mse1Asel Mse1T/TAA Bfa1,Csp61,Nde1-NariAcc1GT/CGAC,Acl1,Cla1,BstB1,Taq1-GG/CGCCAci1,HinP1I HhalBsaHIGR/CGCC Ban1,BsaH1,HaeII,Hha
1.Nari,NiaIVBsaHIGR/CGTC BsaH1,Hga1HpaIIAci1Ncol AfiIIIA/CATGT,BspHI NiaIIIC/CATGGSty1C/CATGG BsaJI,Neo1,NiaIII,Sty1Pci1NiaIIINdel Ase1,Bfa1,Csp61,Mse1-CA/TATGNgoMIVG/CCGGCAge1,BsaW1,BsrF1R/CCGGY,SgrA1BsrF1,HpaIIAva1C/CCGGG,Xma1HpaII.Nci1,ScrFIBsaW
1.BspE1HpaIIBsrF1R/CCGGC,SgrA1BsrFLCac81,HpaII,Nae1AvrII,Spe1,Sty1C/CTAGG,Xba1BfalNhelG/CTAGCSph1,Nsp1NiaIIINiaIIICATGONotlPspOM1Aci1,Eae1,Fnu4H1,Hae川GC/GGCCGCEagl Aci1,BsiE1,Eae1,Eag1,Fnu4H1,HaeIIIEae1Y/GGCCR Aci1,BsiE1,Eae1,Fnu4H1,HaeIIIBsiHKA1GTGCA/C,Bsp1286IGTGCA/C,PstI,SbfI-Nsi1ATGCA/TNiaIII,SphI NiaIIINsplRCATG/YPad BsiEICGAT/CG.PvuI Mse1TTAAT/TAA。
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